Part:BBa_K942018:Design

This part is a mix from the SS of α-mating factor pre-sequence and a consensus non-translational yeast mRNA sequence.

Using this part after the 3’ of a yeast promoter and before the 5’ of any CDS, the user can make a single construct that can yield protein expression in both, E.coli (DE3 strains) and yeast. In our case we used the AOX1 promoter and the CDS for Der f 2 for expression in BL21 STAR, Rossettagami and P.pastoris.

Encrypted in non-translatable sequence after the SS, the T7 promoter (BBa_I712074) and a RBS promote the bacterial expression of the protein. This last feature lets the user transform the same construct for bacterial intracellular expression and extracellular yeast expression. Use this part after the 3’ of a yeast promoter and before the 5’ of any CDS.

About biosecurity This part presents no biosecurity issues apart that any construct after the 3’ of this part could be expressed both in yeast and in bacteria.

This part has an enormous potential due to its adaptability to secrete proteins in yeast and normally produce them in bacteria